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Staff
Curriculum VitaePublicationsResearch
Prof Lodewyk (JLF) Kock
Position   Outstanding Professor
Department   Microbial Biochemical and Food Biotechnology
Address   Biotechnology IB 61
Email  
Telephone   +27-(0)51 4012249/
Office   BTG 28

Short CV

Mission

 

“To do research for its own sake without taking the public into account, is to neglect one’s moral duty as a scientist towards society, especially in a developing country such as South Africa.”

 

“Scientists should ideally pursue the application of their research and should not rest before it provides benefit for the community at large.”

 

“Scientists should always be on the look out for application of their research and should be actively involved in all aspects of its application.”

 

“The links between research and its application are inadequately developed in this country. Consequently, it is important that students are also trained in these aspects in order to become well-balanced scientists who serve the public more effectively.”

 

Kock et al. 1996: SA J Sci  92, 513-514

 

 

 

Background

 

In 1983, Professor J.L.F. Kock established a successful research program in lipid biotechnology (involving fats and oils) at the University of the Free State. In 1991, he discovered novel oxidized fats i.e. oxylipins in yeast. This was highlighted in 1991 by a top international scientific correspondent in the high impact international scientific journal, now called Nature Biotechnology (Impact Factor: 22.355). Since then many international medical researchers and unattached to Kock’s group, confirmed and expanded on Kock’s findings as highlighted in the high impact international medical journal i.e. Clinical Microbiology Reviews (Impact Factor: 11.53; http://cmr.asm.org/cgi/content/full/16/3/517). In 1994, he applied his skills in lipid biotechnology to successfully expose the harmful overuse of edible oils and fats by frying establishments in South Africa. When edible oils and fats are used over and over in frying processes, they start to breakdown through oxidation processes to produce harmful compounds also referred to as oxylipins. Consequently, strict legislation was promulgated in 1996 which made the use of harmful over oxidized oils and fats in food preparation an illegal practice. Kock & team specially concentrated their efforts, through > 100 national workshops, > 100 press releases and various TV and radio broadcasts, on the removal of these harmful overused oil wastes from our poor communities. In 2002, Kock & team published a hypothesis based on 8 years of intensive research i.e. that the intake of unstable polyunsaturated oil (super oxidized soups - SOS), adversely affects poor HIV/AIDS subjects in South Africa. This was supported in 2006 through an extensive epidemiological study by an independent research group (unattached to the Lipid Biotechnology Group) who published these findings in the world’s foremost accredited international nutritional journal i.e. The American Journal of Clinical Nutrition (www.ajcn.org/cgi/reprint/83/5/1193). In 2006, Kock pleas for urgent further research into this matter in the same high impact journal. To combat the consumption of unstable overused oils and fats by the South African community, he established the South African Fryer Oil Initiative (SAFOI) at the UFS in 1994, which actively promotes the use of high quality stable oils and fats and removal of unstable harmful oxidized oil waste from our poor communities (www.uovs.ac.za/myoilguide).

 

Research Impact

 

Research established so far by the Lipid Biotechnology Group resulted in a successful, internationally recognized, unconventional model, showing research groups at tertiary institutions how to become self-sufficient through continuous services to the community (public) and industry while maintaining high quality basic research of own choosing. This model (while still in its infancy of application) was elected as finalist and runner-up in 2004 for the National Award for Achievements in Science and Technology presented by the National Science and Technology Forum (NSTF). Nominees from tertiary and other institutions from across South Africa competed.

 

The model: This innovative balanced model (progression of Kock et al. SA J Sci  92, 513-514, 1996) has not only lead to successful basic research on normal and oxidized oils, using yeasts as reference, but has also successfully been applied to benefit our community especially the poor as well as industry - through the establishment of the South African Fryer Oil Initiative (SAFOI) in 1994.  This research has since 2003 developed into a non-profit routine service of the university ensuring continuous high quality and stable oils to the consumer.

 

These activities have reached a climax over the past 5 years.

 

Since 2003, funds raised from industry are used for high output basic research, student support to present research at international conferences abroad (7 students presenting research at high quality international conference in Berlin, 2006), financial support to international conferences held in Spain, Germany and Finland, departmental research support, etc. 

 

 

Future: To further expand the Kock-model to different fields of research at the UFS and other national and international research institutions. Since the introduction of Kock’s discovery in 1991 (i.e. bioactive oxylipins in yeast) to the international medical arena, many international medical researchers confirmed and expanded this new field of research. This exposed new targets for antifungal action. In 2003, Kock discovered that these oxylipins also serve as lubricants used by fungi to discharge spores for smart dispersal in micron-space. This new field of research, which may find application in nanotechnology, was introduced by Kock in 2006 on invitation to the field of engineering at the 3rd European Conference on Computational Mechanics, Solids, Structures, and Coupled Problems in Engineering, held in Lisbon. Will this discovery have the same impact as that experienced in the medical field?

 

Main Achievements (since 2002)

 (1) 29 articles/chapters published and accepted for publication in high quality accredited scientific journals/books, 3 in other journals and 32 and 22 presentations at international and national scientific conferences respectively. Three M.Sc. (all with distinctions, include 1 Black) and 6 Ph.D. (2 Black, 2 Indian) students successfully completed their studies under Kock’s supervision. Two M.Sc. students will submit their research studies for evaluation end 2006,

(2) skills obtained in lipid research are applied to the benefit of the S.A. community i.e. to terminate the harmful abuse and misrepresentation of edible oils and fats and their consumption by the poor in South Africa. Over the past five years 21 articles were written and published by popular press while 14 interviews over radio and TV programs were broadcasted concerning Kock’s research and application in community. This was also broadcasted globally over numerous stations via Voice of America and Reuters TV. In addition, Kock & team issued 18 newsletters and presented 33 workshop across South Africa aimed at Health Departments as well as the foodservice and oil industries. Quality control for various oil industries is performed routinely as a non-profit university service (www.uovs.ac.za/myoilguide),

(3) received the American Society for Microbiology (ASM) MIRCEN Award in 2002,

(4) runner-up in 2004 for the National Award for Achievements in Science and Technology presented by the National Science and Technology Forum (NSTF),

(5) appointed Commissioner for South Africa of the International Commission on Yeasts (ICY, 2004),

(6) present UNESCO Directors’ Report as South African MIRCEN Director in 2002, at the National Academy of Science, Washington, USA. Act also as Rapporteur at this event,

(7) convenor: NRF evaluation (rating) panel for Microbiology and Plant Pathology (2006-2007),

(8) students under Kock’s supervision: (i) Ph.D. student (Gontse Morakile) successfully complete study which contributed to the production of the world’s best lager beer by South African Breweries; (ii) Ph.D. student (Sechaba Bareetseng) wins award for young scientists for best presentation at the International Specialised Symposium on Yeasts (ISSY 2003), Budapest, Hungary, 2003; (iii) M.Sc. student (Olihile Sebolai) awarded Senates and Faculty Medals for best M.Sc. dissertation in 2005. In addition 4 more awards were presented for this achievement. This was broadcasted also on SABC TV News, Reuters TV and Voice of America globally, national radio and various newspapers. Minister Naledi Pandor congratulated student, rector and Kock on achievement in 2005. Highlighted as SA Young Achiever in CEO magazine (Celebrating Excellence in Organisations), Vol. 4 No. 4 – 2005. Student was presented with the Free State Premier Excellence Award on 22 April 2006; (iv) Ph.D. student (Catrine Strauss), only South African selected to present talk at 24th International Specialised Symposium on Yeasts (ISSY 2005), Valencia, Spain, 2005 and selected to present talk at the XXII International Conference on Yeast Genetics and Molecular Biology (Bratislava, Slovak Republic),

(9) National Research Foundation (NRF) highlights Kock’s research in April 2005 edition of News@NRF,

(10) Kock’s research on cover page of all 2006 issues of top accredited European yeast journal i.e. FEMS Yeast Research as well as an issue of SA Journal of Science,

(11) NRF ratings: 1986 - P; 1990 - B2; 1994 – B2; 1998 – B2; 2003 – B1,

(12) awarded outstanding professor by UFS since 1998,

(13) Kock’s discovery of oxidized lipids (oxylipins) in yeasts was highlighted by Bernard Dixon in Biotechnology (1991), now called Nature Biotechnology (Impact Factor = 22.355).  In 2003, this discovery was independently recognized and confirmed by Noverr et al. (2003; University of Michigan Medical School, Ann Arbor, Michigan) in the high impact scientific medical journal i.e. Clinical Microbiology Reviews (Impact Factor= 11.53). These authors referred to 14 publications in which Kock was involved (include 12 from Kock’s laboratory), http://cmr.asm.org/cgi/content/full/16/3/517,

(14) uncover used oil scandal in collaboration with Carte Blanche (investigative journalism TV-show broadcasted on 1/8/2004). This was also published in Huisgenoot and You magazines in 2004,

(15) Kock’s hypothesis (Kock et al., 2002 SA J Sci 98: 413-) that unstable polyunsaturated oils, distributed to the poor Black communities of S.A., adversely affects HIV/AIDS subjects was confirmed in an extensive epidemiological study by an independent research group at North West University. Findings published in foremost accredited international nutritional journal (The American Journal of Clinical Nutrition, Impact factor = 5.1; www.ajcn.org/cgi/reprint/83/5/1193),

(16) successfully developed and applied the Steward’s Principle (a self-regulatory system to terminate oil abuse and harmful oil waste distribution to the poor), across S.A.,

(17) already contracted 5 oil manufacturing/distributing companies (i.e. Felda Bridge Africa, Refill Oils, PSS Oils, Rein Oils and Ilanga Oils) who wants to display UFS seal of approval on their products,

(18) organizing committee: (1) 22nd International Specialised Symposium on Yeasts.  Pilanesberg National Park, South Africa, 25 – 28 March 2002; (2) co-organizer of the 2nd International Symposium on Non-Mammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins held in Berlin, Germany in May 2006,

(19) invited talks: (1) International Symposium on Recent BioMedical Advances in Eicosanoid Research. Berlin, 2002; (2) 23rd International Specialised Symposium on Yeasts (ISSY23) Budapest, 2003; (3) 18th Congress of the South African Genetics Society/South African Microbiology Society, Stellenbosch, 4-7 April, 2004; (4) 11th International Congress on Yeasts, Rio de Janeiro, Brazil, 2004; (5) 2nd International Conference on Non-Mammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins held in Berlin, Germany, 2006; (6) III European Conference on Computational Mechanics Solids, Structures and Coupled Problems in Engineering, Lisbon, Portugal, June, 2006,

(20) acting chair at conferences: (1) 22nd International Specialised Symposium on Yeasts. Pilanesberg National Park, South Africa, 2002; (2) 12th Biennial Congress of the South African Society for Microbiology, University of the Free State, Bloemfontein, 2002; (3) 11th International Congress on Yeasts, Rio de Janeiro, Brazil, 2004; (4) 2nd International Conference on Non-Mammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins, Berlin, Germany, 2006,

(21) member of International Advisory Committees: (1) 23rd International Specialised Symposium on Yeasts. Budapest, Hungary, 2003; (2) 2nd International Conference on Non-Mammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins. Berlin, Germany, 2006,

(22) invited to participate in the preparation of the internationally acknowledged monographic series on yeasts: “The Yeasts, a taxonomic study, Fifth Edition” which will be published in 2007.  This is the most widely recognized literature on yeast taxonomy.

 

 

Acknowledgements

 

The UFS management, students as well as national and international collaborators for making all of the above possible.

 

Successful M.Sc. students under Kock’s supervision since 2002

1.             Miss T. Venter – M.Sc. (Reg. 2002, pass with distinction 2004; S2A3 medal & Van der Walt prize for best M.Sc. on yeasts)

Title: The production of citric acid by Yarrowia lipolytica when cultivated on edible and waste fats.

Study leader: Prof. J.L.F. Kock

Co-study leader: Prof. M. Smit

2.             Miss M. Kalorizas – M.Sc. (Reg. 2002, pass with distinction 2004)

Title:  The value of lipid profiling and RFLP analysis in the taxonomy of the genus Kluyveromyces.

Study leader: Prof. J.L.F. Kock

Co-study leader: Dr. C.H. Pohl; Dr. J. Albertyn

3.             O.M. Sebolai – M.Sc. (Reg. 2003; passed with distinction 2005 & awarded 6 prizes: Black)

Title: The lipid composition of the yeast genus Saccharomycopsis Schiönning.

Study leader: Prof. J.L.F. Kock

Co-study leaders: Profs M.S. Smit; P.W.J. van Wyk

  

Successful Ph.D. students under Kock’s supervision since 2002

1.             Mr. G.I.  Morakile – Ph.D. (Reg. 1999, Pass 2002: Black)

Title: Preservation, Inoculum Development and Quality Management of Yeasts in the   Brewing Industry.

                Study Leader: Prof. J.L.F. Kock

                Co-study Leader: Prof. B.C. Viljoen

2.             Miss D.P. Smith – Ph.D. (Pass 2003).

Title: 3-Hydroxy oxylipins in the yeast families Lipomycetaceae and Dipodascaceae.

Study Leader: Prof. J.L.F. Kock

Co-study Leader: Prof. P.W.J. van Wyk; Dr. E. van Heerden

3.             Miss M. Joseph – Ph.D. (Pass 2004: Indian)

                Title:  Quality management and fungal transformation in the edible oil industry.

Study Leader: Prof. J.L.F. Kock

Co-study Leaders: Drs E. van Heerden; A. Hugo

4.             Miss S. Tarr – Ph.D. (Pass 2005: Indian)

                Title: Yeasts from Lesotho – their classification and possible applications.

Study Leader: Prof. J.L.F. Kock

Co-study Leaders: Prof. M.S. Smit; Dr. J. Albertyn

5.             Mr. S. Bareetseng – Ph.D. (Passed 2005: Black)

                Title: Lipids and ascospore morphology in yeasts.

Study Leader: Prof. J.L.F. Kock.

Co-study Leaders: Prof. P.W.J. van Wyk; Dr. C.H. Pohl

6.             Miss CJ Strauss – Ph.D. (Pass 2006)

                Title: The role of lipids in the flocculation of Saccharomyces cerevisiae.

Study Leader: Prof. J.L.F. Kock

Co-study Leaders: Prof. P.W.J. van Wyk; Dr. E.J. Lodolo (SA Breweries)

 

 

Area of Expertise

Objectives of research:

1. Training of skilled manpower in the field of lipid biotechnology

2. To improve food quality in South Africa by effectively reducing the consumption of hazardous over-oxidised and adulterated frying oils and fats by especially poor communities

3. To investigate the production of animal feed, valuable oils and protein from frying oils and fats, their wastes and other sources

4. Maintenance and expansion of an extensive yeast culture collection which serves as basis for the development of lipid biotechnological processes, taxonomic studies and deposit for industry.

 

Courses Presented

BLG 114 (Molecular and Cell Biology)

MKB 214 (Introduction to Microbiology)

MKB 334 (Microbial Eukaryotic Diversity and Ecology)

IQM 242 (Industrial Quality Management)

MKB 614 (Advanced Techniques)

MKB 634 (Microbial Diversity)

Publications (Short List)

Publications in accredited scientific journal of repute (since 2002)

All publications in this section appear in journals that are indexed by the Institute for Scientific Information (ISI) in the USA..

1.             Morakile, G., Pretorius, E.E., Pohl, C.H. and Kock, J.L.F. (2002) Comparison of preservation methods for the maintenance of brewing yeast. S.A. Journal of Science 98: 23 –24.

2.             Morakile, G., Kock, J.L.F. and Botes, P.J. (2002) The use of fatty acid and sterol analyses as quality control methods in the brewing industry. Journal of the Institute of Brewing 108(2): 160- 163.

3.             Morakile, G., Kock, J.L.F., Van Zyl, W., Pohl, C.H. and Viljoen, B.C. (2002) Differentiation of brewing and related yeasts based on PCR amplification and restriction fragment length polymorphism of ribosomal DNA.  Journal of the Institute of Brewing 108(2): 164- 168.

4.             Kock, J.L.F., Pohl, C.H. and Venter, A. (2002) Super oxidized soups and the health risks to poor South Africans.  S.A. Journal of Science 98: 413-414.

5.             Smith, D.P., Kock, J.L.F., Van Wyk, P.W.J., Pohl, C.H., Van Heerden, E., Botes, P.J. and Nigam, S. (2003) Oxylipins and ascospore morphology in the ascomycetous yeast genus Dipodascus. Antonie van Leeuwenhoek 83: 317-325.

6.             Strauss, C.J., Kock, J.L.F., Van Wyk, P.W.J., Viljoen, B.C., Botes, P.J., Hulse, G. and Nigam, S. (2003) Inverse flocculation patterns in Saccharomyces cerevisiae UOFS Y-2330. Journal of the Institute of Brewing 109 (1): 3-7.

7.         Kock, J.L.F., Strauss, C.J., Pohl, C.H. and Nigam, S. (2003) Invited Review: The distribution of 3-hydroxy oxylipins in fungi.  Prostaglandins & Other Lipid Mediators 71: 85-96.

8.             Venter, T., Kock, J.L.F., Botes, P.J., Smit, M.S., Hugo, A. and Joseph, M. (2004) Acetate enhances citric acid production by Yarrowia lipolytica when grown on sunflower oil. Systematic and Applied Microbiology 27: 135 – 138.

9.             Bareetseng, A.S., Kock, J.L.F., Pohl, C.H., Pretorius, E.E. and Van Wyk, P.W.J. (2004) Variation in functional ascospore parts in the ascomycetous yeast Dipodascopsis uninucleata. Antonie van Leeuwenhoek 85: 187-189.

10.           Sebolai, O.M., Kock, J.L.F., Pohl, C.H. and Botes, P.J. (2004) Report on a novel 3-hydroxy oxylipin cascade in the yeast Saccharomycopsis synnaedendra. Prostaglandins and Other Lipid Mediators 74(1-4): 139-146.

11.           Bareetseng, A.S., Kock, J.L.F., Pohl, C.H., Pretorius, E.E., Strauss, C.J., Botes, P.J., Van Wyk, P.W.J. and Nigam, S. (2004) Mapping 3-hydroxy oxylipins on ascospores of Eremothecium sinecaudum. Antonie van Leeuwenhoek 86: 363-368.

12.           Kock, J.L.F., Strauss, C.J., Pretorius, E.E., Pohl, C.H., Bareetseng, A.S., Botes, P.J., van Wyk, P.W.J., Schoombie, S.W. and Nigam, S. (2004) Revealing yeast spore movement in confined space. S.A. Journal of Science 100 (5/6): 237-240. (Include cover page).

13.           Strauss, C .J., Kock, J.L.F., Viljoen, B.C., Botes, P.J., Hulse, G. and Lodolo, E. (2004)  Lipid turnover during inverse flocculation in Saccharomyces cerevisiae UOFS Y-2330. Journal of the Institute of Brewing 110(3): 207-212.

14.           Bareetseng, A.S., Kock, J.L.F., Pohl, C.H., Pretorius, E.E. and Van Wyk, P.W.J. (2005) Uncovering the first double brimmed hat-shaped ascospores in Ambrosiozyma platypodis Van der Walt. Antonie van Leeuwenhoek 87: 169- 170.

15.           Joseph, M., Kock, J.L.F., Pohl, C.H., Botes, P.J., van Heerden, E. and Hugo, A. (2005) Acetate-enhanced polymerized triacylglycerol utilization by Mucor circinelloides. World Journal of Microbiology and Biotechnology 21: 97-99.

16.           Bareetseng, A.S., Kock, J.L.F., Pohl, C.H., Pretorius, E.E., Botes, P.J., Van Wyk, P.W.J. and Nigam, S. (2005) The presence of 3-hydroxy oxylipins on surfaces of hat-shaped ascospores of Ascoidea africana Batra & Francke-Grosmann. Canadian Journal of Microbiology 51(1): 99-103.

17.           Sebolai, O.M., Kock, J.L.F., Pohl, C.H., Botes, P.J., Strauss, C.J., Van Wyk, P.W.J. and Nigam, S. (2005) The presence of 3-hydroxy oxylipins on the ascospore surfaces of some species representing Saccharomycopsis Schionning. Canadian Journal of Microbiology 51(7): 605-612.

18.           Van Heerden, A., Kock, J.L.F., Botes, P.J., Pohl, C.H., Strauss, C.J., Van Wyk, P.W.J. and Nigam, S. (2005) Ascospore release from bottle-shaped asci in Dipodascus albidus. FEMS Yeast Research 5(12): 1185-1190.

19.           Ciccoli, R., Sahi, S., Singh, S., Prakash, H., Zafiriou, M-P., Ishdorj, G., Kock, J.L.F. and Nigam, S. (2005) Oxygenation by cyclooxygenase-2 (COX-2) of 3-Hydroxyeicosa-tetraenoic acid (3-HETE), a fungal mimetic of arachidonic acid, produces a cascade of novel bioactive 3-hydroxy-eicosanoids. Biochemistry Journal 390: 737-747.

20.           Strauss, C.J., Kock, J.L.F., Van Wyk, P.W.J., Lodolo, E.J., Pohl, C.H. and Botes, P.J. (2005) Bioactive oxylipins in Saccharomyces cerevisiae. Journal of the Institute of Brewing 111(3): 304-308.

21.           Kock, J.L.F., Kock, J.L.F. (Jr), Strauss, C.J. and Pohl, C.H. (2005) Letter: Aspirin: the anti-inflammatory, antifungal, antibacterial agent? S.A. Journal of Science 101: 494-495.

22.           Bareetseng, A.S., Kock, J.L.F., Pohl, C.H., Pretorius, E.E., Strauss, C.J., Botes, P.J., Van Wyk, P.W.J. and Nigam, S. (2006) Mapping the distribution of 3-hydroxy oxylipins in the ascomycetous yeast Saturnispora saitoi. Systematic and  Applied Microbiology 29:446-449.

23.           Leeuw, N.J., Kock, J.L.F., Pohl, C.H., Bareetseng, A.S., Sebolai, O.M., Joseph, M., Strauss, C.J., Botes, P.J., Van Wyk, P.W.J. and Nigam, S. (2006) Oxylipin covered ascospores of Eremothecium coryli. Antonie van Leeuwenhoek 89(1): 91-97.

24.           Strauss, C.J., Van Wyk, P.W.J., Lodolo, E.J., Botes, P.J., Pohl, C.H., Nigam, S.  and Kock, J.L.F. (2006) Oxylipin associated co-flocculation in yeasts. Journal of the Institute of Brewing 112(1): 66-71.

25.           Kock, J.L.F. (2006) Letter: Is the consumption of oxidised fats contributing to HIV/AIDS progression in South Africa? The American Journal of Clinical Nutrition 84(3): 666.667.

26.           Pohl, C.H., Kock, J.L.F., Van Wyk, P.W.J. and Albertyn, J. (2006) Cryptococcus anemochoreius sp nov, a new anamorphic basidiomycetous yeast isolated from the atmosphere in central South Africa. International Journal of Systematic and Evolutionary Microbiology 56: 2703-2706.

27.           Ncango, D.M., Pohl, C.H., Sebolai, O.M., Botes, P.J., Strauss, C.J., Joseph, M., van Wyk P.W.J., Nigam, S. and Kock, J.L.F. (2006) Oxylipin-coated hat-shaped ascospores of Ascoidea corymbosa. Canadian Journal of Microbiology 52(11): 1046-1050.

28.           Van Heerden, A., Van Wyk, P.W.J., Botes, P.J., Pohl, C.H., Strauss, C.J., Nigam, S. and Kock, J.L.F. (2007) The release of elongated, sheathed ascospores from bottle-shaped asci in Dipodascus geniculatus. FEMS Yeast Research 7: 173-179.

29.           Leeuw, N.J., Swart, C.W., Ncango, D.M., Pohl, C.H., Sebolai, O.M., Strauss, C.J., Botes, P.J., van Wyk, P.W.J., Nigam, S.  and Kock, J.L.F. (2007) Acetylsalicylic acid as antifungal in Eremothecium and other yeasts. Antonie van Leeuwenhoek  91: 393-405.

30.           Sebolai, O.M., Pohl, C.H., Botes, P.J., Strauss, C.J., van Wyk, P.W.J., Botha, A. and Kock, J.L.F.  (2007) 3-Hydroxy fatty acids found in capsules of Cryptococcus neoformans. Canadian Journal of Microbiology 53 (6): 809-812.

31.           Strauss, C.J., van Wyk, P.W.J., Lodolo, E.J., Botes, P.J., Pohl, C.H., Nigam, S. and Kock, J.L.F. (2007) Mitochondrial associated yeast flocculation – the effect of acetylsalicylic acid. Journal of the Institute of Brewing  113(1): 42-47.

32.           Kock, J.L.F., Sebolai, O.M., Pohl, C.H., van Wyk, P.W.J. and Lodolo, E.J. (2007) Oxylipin studies expose aspirin as antifungal. FEMS Yeast Research (In Press).

 

Chapters in accredited scientific books of repute

33.           Kock, J.L.F., Strauss, C.J., Pohl, C.H., Van Wyk, P.W.J. and Botes, P.J. (2006) Yeast Biomechanics. Proceedings: III European Conference on Computational Mechanics Solids, Structures and Coupled Problems in Engineering. Lisbon, Portugal, 5-8 June 2006, Eds. C.A. Mota Soares et al. p. 725. ISBN-10 1-4020-4994-3 (HB) & ISBN-13 978-1-4020-4994-1 (HB). Springer, The Netherlands.

 

Publications in other journals

1.             Kock, J.L.F., Joseph, M., Hugo, A., Venter, A., Groenewald, P. and Pohl, C.H. (2002) Are the current South African food regulations sufficient to combat edible oil and fat abuse? S.A. Grain August: 58-61.

2.             Venter, A., Kock, J.L.F. and Pohl, C.H. (2003) Abused cooking oil: a ticking time bomb? Health & Hygiene April: 22-23.

3.             Kock, J.L.F. (2005) A solution to oil abuse in South Africa. RB Magazine April: 22-. COVER STORY.

 

International conferences

 

22nd International Specialized Symposium on Yeasts (ISSY), Pilanesberg National Park, South Africa, March, 2002.

1. Title: Lipids and fungal taxonomy, J.L.F. Kock, C.J. Strauss, D.P. Smith, C.H. Pohl, S. Nigam.

2. Title: 3-Hydroxy oxylipins in yeasts, D.P. Smith, J.L.F. Kock, P.W.J. van Wyk, C.H. Pohl, S. Nigam.

3. Title: Comparison of preservation methods in the maintenance of brewing yeast, G. Morakile, E.E. Pretorius, C.H. Pohl, J.L.F. Kock.

4. Title: Identification and characterisation of yeasts isolated from doro and mukumbi, two Zimbabwean traditional alcoholic beverages, A. Mpofu, J..M. Bvochora, E.E. Pretorius, C. Pohl, E. Nyakudya, R. Zvauya, J.L.F. Kock.

 

International Symposium on Recent Biomedical Advances in Eicosanoid Research, Berlin, Germany, August 22-24, 2002.

5. Title: The distribution of 3-hydroxy oxylipins in fungi: an overview, J.L.F. Kock, C. Strauss, D.P. Smith, C.H. Pohl, P.W.J. van Wyk, S. Nigam (Invited Talk).

 

International Conference on Electron Microscopy (ICM), Durban, 2002.

6.Title:    Ultrastructure of oxylipins involved in yeast cell flocculation, P.W.J. van Wyk, J.L.F. Kock, S. Nigam.

 

Managing Fats and Oil Supplies for Human Needs, Strasbourg, France, November, 2002.

7. Title: Fungal biotransformation in the edible oil industry, M. Joseph, J.L.F. Kock.

 

Plant Oxylipins – 2nd Joint Symposium, Göttingen, Germany, June 12 – 14, 2003.

8. Title: 3-Hydroxyoxylipins, a group of novel fungal eicosanoids, S. Nigam, R. Deva, R. Ciccoli, I. Ivanov, H. Ruter, S. Sahi, J.L.F. Kock, et al.

9. Title: The distribution of 3-hydroxyoxylipins in fungi and its implications, J.L.F. Kock, C.J. Strauss, S. Nigam. 

 

23rd International Specialized Symposium on Yeasts (Interactions between yeasts and other organisms), Budapest, Hungary, August, 2003.

 

10. Title: Lipids and yeast taxonomy, Kock, J.L.F. (Invited talk).

11. Title: The value of RFLP analysis and lipid composition in the taxonomy of the genus Kluyveromyces,  Kalorizas, M., Kock, J.L.F., Pohl, C.H., Botes, P.J., Albertyn, A.

12. Title: Variation in functional ascospore parts in the ascomycetous yeast Dipodascopsis uninucleata. (Awarded 1st Prize), Bareetseng, A.S., Kock, J.L.F., Pohl, C.H., Pretorius, E.E., Van Wyk, P.W.J. 

13. Title: Lipomycetaceous yeasts from the Lesotho highlands, Tarr, S., Kock, J.L.F., Pretorius, E., Botes, P.

14. Title: The influence of acetate on citric acid production by Yarrowia lipolytica when cultivated on sunflower oil, Venter, T., Kock, J.L.F., Smit, M.S., Hugo, A., Joseph, M.

15. Title: Inverse flocculation patterns in Saccharomyces cerevisiae UOFS Y 2330, Strauss, C.J., Kock, J.L.F., van Wyk, P.W.J., Hulse, G., Nigam, S. 

 

11th International Congress on Yeasts (ISSY), Rio de Janeiro, Brazil, August, 2004.

16. Title: Yeast mechanics in micron-space, Kock, J.L.F. et al. (Invited talk).

17. Title: Evidence for oxylipin-lectin- mediated yeast cell aggregation mechanism using electron microscopy and laser confocal microscopy, Van Wyk, P.W.J., Strauss, C.J., Kock, J.L.F. et al.

 

24th International Specialized Symposium on Yeasts (24th ISSY). Oropesa del Mar, Castellon, Spain, 28 September – 2 October, 2005.

18. Title: Message in a bottle, Van Heerden, A., Kock J.L.F. et al.

19. Title: Cell walls and yeast flocculation: the role of oxylipins, Strauss, C.J., Kock, J.L.F. et al.

 

2nd International Conference on Nonmammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins, Berlin, Germany, 24 – 27 May, 2006.

20. Title: Yeast biomechanics: Message in a bottle, Van Heerden, A., Strauss, C.J., Pohl, C.H., van Wyk, P.W.J., Nigam, S., Kock, J.L.F.

21. Title: Oxylipin covered ascospores of Eremothecium coryli, Leeuw, N.J., Pohl, C.H.,  Bareetseng, A.S., Sebolai, O.M., Joseph, M., Strauss, C.J., Botes, P.J., van Wyk, P.W.J., Nigam, S., Kock, J.L.F.

22. Title: Forced release of hat-shaped ascospores in Ascoidea corymbosa, Ncango, M.D., Pohl, C.H., Sebolai, O.M., Botes, P.J., Strauss, C.J., Joseph, M., van Wyk, P.W.J., Nigam, S., Kock, J.L.F.

23. Title: Phenotypic variation in oxylipin-producing Dipodascopsis uninucleata, Goldblatt, M.E., Botes, P.J., van Wyk, P.W.J., Strauss, C.J., Nigam, S., Pohl, C.H., Kock. J.L.F.

24. Title: 3-Hydroxy oxylipins associated with Candida magnolia, Swart, C.W., Botes, P.J., van Wyk, P.W.J., Strauss, C.J., Nigam, S., Pohl, C.H., Kock, J.L.F.

25. Title: Message in a bottle: 3-hydroxy oxylipins and yeast biomechanics, Kock, J.L.F., Strauss, C.J., Pohl, C.H., van Wyk, P.W.J., Nigam, S. (Invited talk).

26. Title: Yeast flocculation and role of oxylipins, Strauss, C.J., van Wyk, P.W.J., Lodolo, E.J., Botes, P.J., Nigam, S., Kock, J.L.F. (Invited talk).

27. Title: Application of electron and confocal laser scanning microscopy in observing oxylipins in fungal cells, van Wyk, P.W.J., Strauss, C.J., Kock, J.L.F., Nigam, S. (Invited talk).

28. Title: Oxygenation by COX-2 and 5-, 12- and 15- lipoxygenases of 3-hydroxy-eicosatetraenoic acid, a fungal mimetic of AA, produces a cascade of novel bioactive 3-hydroxy-eicosanoids, Ciccoli, R., Sahi, S., Deva, R., Singh, S., Zafiriou, M-P., Kock, J.L.F.,  Nigam, S.

29. Title: Inverse flocculation in Sacch. cerevisiae, Strauss, C.J., Kock, J.L.F., van Wyk, P.W.J., Botes, P.J., Nigam, S.

30. Title: A novel aspirin-sensitive 3-hydroxy oxylipin release mechanism in Cryptococcus neoformans, Sebolai, O.M., Pohl, C.H., Botes, P.J., Strauss, C.J., van Wyk, P.W.J., Nigam, S.

 

International Specialized Symposium on Yeasts (ISSY 25), Hanasaari, Espoo, Finland, June 18-21, 2006.

31. Title: Yeast “nanobots”, Pohl, C.H., Strauss, C.J., van Wyk, P.W.J., Kock, J.L.F.

 

III European Conference on Computational Mechanics, Solids, Structures and Coupled Problems in Engineering, Lisbon, Portugal, June, 2006.

32. Title: Yeast Biomechanics, Kock, J.L.F., Strauss, C.J., Pohl, C.H., Van Wyk, P.W.J., Botes, P.J. (Invited Talk)

 

 

National conferences

 

12th Biennial Congress of the South African Society for Microbiology, University of the Free State, Bloemfontein, April, 2002.

1. Title:  Immunocytochemical detection of 3-hydroxy oxylipins in ascomycetous yeasts, Smith, D.P., Kock, J.L.F., van Wyk, P.W.J., Pohl, C.H., Nigam, S.

2. Title:  Ascospore morphology and lipid turnover in the yeast Nadsonia fulvescens, Bareetseng, A.S., Kock, J.L.F., Pohl, C.H., van Wyk, P.W.J.

3. Title:  Lipid turnover during growth and development of Mucor genevensis, Pohl, C.H., Botha, A., Kock, J.L.F., Coetzee, D.J., Botes, P.J., Nigam, S.

4. Title:  The presence of oxylipins in yeast flocculation, Kock, J.L.F., Strauss, C.J., Venter, P., Smith, D.P., van Wyk, P.W.J., Botes, P.J., Pohl, C.H., Nigam, S.

5. Title: The influence of the environment on the lipid content of the yeast genus Kluyveromyces and its taxonomic implications, Kalorizas, M., Kock, J.L.F., Pohl, C.H.

6. Title: The biotransformation of overused frying fats by indigenous isolates of mucoralean fungi, Anelich, L.C.M.E., Kock, J.L.F., Botha, A., Roux-Van der Merwe, M.P.

7. Title: Fungal biotransformation in the edible oil industry, Joseph, M., Tarr, S., Pelesane, T.R., Kock, J.L.F., Pohl, C.H., Viljoen, B.C.

8. Title: Differentiation of brewing and related yeasts based on PCR amplification and restriction fragment length polymorphism of ribosomal DNA, Morakile, G., Pohl, C.H., Kock, J.L.F., van Zyl, W.B., Viljoen, B.C.

 Ibuya Congress: ICC Durban, South Africa, 22 March, 2002.

 9. Title: Recycling: giving back to the community. Venter, A., Kock, J.L.F., Pohl, C.H.

 

Environmental Health Conference, ICC Durban, 19 November, 2002.

10. Title: The development of an educational program to address the distribution of potentially hazardous cooking oils to the informal sector. Venter, A., Kock, J.L.F., Pohl, C.H. 

 

18th Congress of the South African Genetics Society/South African Microbiology Society, Stellenbosch, 4-7 April, 2004.

11. Title: Fungal transformations in the frying oil industry, Joseph, M., Kock, J.L.F., Pohl, C.H., Viljoen, B.C.

12. Title: Variation in functional ascospore parts, Bareetseng, A.S., Kock, J.L.F., Pohl, C.H., Pretorius, E.E., van Wyk, P.W.J.

13. Title: The presence of 3-hydroxy palmitic acid in Saccharomycopsis malanga, Sebolai, O.M., Kock, J.L.F., Pohl, C.H., Smith, D.P., Botes, P.J., Pretorius, E.E., van Wyk, P.W.J., Nigam, S.

14. Title: Lipomycetaceous yeasts from Southern Africa, Tarr, S., Kock, J.L.F., Pretorius, E.E., Pohl, C.H., Albertyn, J.

15. Title: Yeast mechanics in micron-space II, Kock, J.L.F. (Invited talk and co-chair of session).

16. Title: Inverse flocculation patterns in Saccharomyces cerevisiae UOFS Y-2330, Strauss, C.J., Kock, J.L.F., van Wyk, P.W.J., Viljoen, B.C., Botes, P.J., Nigam, S.

 

44th Annual Conference of the Microscopy Society of Southern Africa, Pietermaritzburg, Proceedings 35, 36, 2005.

17. Title: Macro-messages in a micro-bottle, van Wyk, P.W.J., Kock, J.L.F., van Heerden, A., Strauss, C.J., Pohl, C.H., Schoombie, S.W., Nigam, S.

 

The 14th Biennial Congress of the South African Society for Microbiology, CSIR, Pretoria, 9-12 April, 2006.

18. Title: Yeast biomechanics: Message in a bottle, Van Heerden, A., Strauss, C.J., Pohl, C.H., van Wyk, P.W.J., Nigam, S., Kock, J.L.F.

19. Title: Yeast biomechanics: The function of spindle-shaped ascospores in Eremothecium coryli, Leeuw, N.J., Pohl, C.H., Bareetseng, A.S., Sebolai, O.M., Joseph, M., Strauss, C.J., Botes, P.J., van Wyk, P.W.J., Nigam, S., Kock, J.L.F.

20. Title: Yeast biomechanics: Forced release of hat-shaped ascospores in Ascoidea corymbosa, Ncango, M.D., Pohl, C.H., Sebolai, O.M., Botes, P.J., Strauss, C.J., Joseph, M., van Wyk, P.W.J., Nigam, S., Kock, J.L.F.

21. Title: Yeast biomechanics: Phenotypic variation in oxylipin-producing Dipodascopsis uninucleata, Goldblatt, M., Botes, P.J., van Wyk, P.W.J.,  Strauss, C.J., Nigam, S., Pohl, C.H., Kock, J.L.F.

22. Title: 3-OH oxylipins associated with Candida magnolia, Swart, C.W., Botes, P.J., van Wyk, P.W.J., Strauss, C.J., Nigam, S., Pohl, C.H., Kock, J.L.F. (Won second prize for best poster overall).

 

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